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KMID : 0367420050480080871
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Journal of Korean Pediatric Society
2005 Volume.48 No. 8 p.871 ~ p.876
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Tumor Necrosis Factor and Lymphotoxin-alpha Gene Polymorphism in Korean Children with Type 1 Diabetes
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Suh Jin-Soon
Park So-Young
Jung Min-Ho
Suh Byung-Kyu
Lee Byung-Churl
Kim Tai-Gyu
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Abstract
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Purpose: Recently, it was reported that tumor necrosis factor(TNF) and lymphotoxin-alpha(LT-alpha) gene regions might be a susceptible loci to type 1 diabetes in Japanese. The purpose of this study was to investigate the association of TNF and LT-alpha gene polymorphisms with disease susceptibility in Korean children with type 1 diabetes.
Methods: Forty-nine Korean children with type 1 diabetes(29 girls and 20 boys) and 94 healthy Koreans were investigated in this study. Genotyping for -857T/C polymorphism in the TNF promoter region and LT-alpha gene polymorphism were performed by PCR-RFLP(restriction fragment length polymorphism). TNF promoter -1031C/T polymorphism was detected by allele-specific PCR.
Results: The distribution of the -857T/C and -1031C/T genotype in the TNF promoter region was not different between diabetic children and the controls. The frequency of TT genotype in the distribution of TNF -1031C/T polymorphism in diabetic children with diabetic ketoacidosis(DKA) at diagnosis was significantly lower than those without DKA(P<0.05). No significant difference in the distribution of LT-alpha gene polymorphism was observed between diabetic children and the controls. There was no association between clinical characteristics of type 1 diabetes and LT-alpha gene polymorphisms.
Conclusion: These results suggest that TNF promoter -857T/C and LT-alpha gene polymorphisms are not associated with susceptibility to type 1 diabetes in Korean children. TNF promoter -1031C/T polymorphism might be related to clinical manifestations(DKA) of type 1 diabetes.
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KEYWORD
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Type 1 diabetes, Gene, Polymorphism, Tumor necrosis factor, Lymphotoxin-a
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